Effectiveness Of Incision Wound Healing Of Pandanus Amaryllifolius Roxb In Wistar Rats
DOI:
https://doi.org/10.51601/ijhp.v2i2.33Abstract
The incision is a condition of tissue discontinuity that is commonly encountered with the risk of
prolonged healing time, imperfect wound healing, infection, etc. Assessment of the wound healing
activity of the incision is the purpose of this research, which includes an examination of wound
contraction, the thickness of collagen fibers, and the number of new blood vessels formation. In
total, 25 rats will be divided into 5 test groups, each consisting of 5 rats; the control group (cream
base treated), standard group (Povidone Iodine), followed by the treated group with 10%, 20%,
and 30% pandan leaf extract ointment. The preliminary screening of phytochemical analysis
showed the presence of alkaloids, saponins, flavonoids, tannins, triterpenoids, and steroids and
glycosides, with the total content of phenols and flavonoids from the ethanolic extract of Pandan
Wangi is 20.95 GAE mg/gram extract, and 23.57 TAE mg/gram extract. Wound contraction
examination result showed a significant difference between the treatment groups on day 16 and a
sign the pairs of groups after being given the ointment with each concentration (p <0.05),
especially in the group with 30% fragrant leaves extract and the standard group. Examination of
new blood vessels showed a significant difference (p <0.05), especially with 30% of the leaves
extract ointment. There was also a significant difference in collagen density in the group given
pandanus ethanol ointment, especially at the 30% pandanus extract content against the group that
was only given the ointment base. This research strongly suggests that the fragrant pandan leaf
extract ointment is effective in healing incisional wounds and promises the potential for further
studies on the pharmacological aspects.
References
Aini R, Mardiyaningsih A. Pandan leaves extract (Pandanus amaryllifolius Roxb) as a food preservative. Jurnal
Kedokteran dan Kesehatan Indonesia. 2016 Oct 17;7(4):166-73.
Ambreen, Madieha, and Safdar Ali Mirza. "Evaluation of anti-inflammatory and wound healing potential of
tannins isolated from leaf callus cultures of Achyranthes aspera and Ocimum basilicum." Pakistan journal of
pharmaceutical sciences 33 (2020).
Angkasa C, Angkasa A, Lister IN, Girsang E, Fachrial E. Effectiveness Test on Centella Asiatica Extract on
Increasing Elasticity Level on Male Mus Musculus. American Scientific Research Journal for Engineering,
Technology, and Sciences. 2019 Aug 10;58(1).
Asrade S, Geta M, Fentahun E (2020). "In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of
% Etanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice". Volume 2020, DOI:
https://doi.org/10.1155/2020/9645792.
Bhuyan, B., & Sonowal, R. (2021). AN OVERVIEW OF Pandanus amaryllifolius Roxb. ex Lindl. AND ITS
POTENTIAL IMPACT ON HEALTH. Current Trends in Pharmaceutical Research, 8(1).
Bouyahya, A. et al. (2016) 'Determination of Phenolic Contents, Antioxidant and Antibacterial Activities of
Strawberry Tree (Arbutus unedo L.) Leaf Extracts,' British Biotechnology Journal, 14(3), pp. 1–10. DOI:
9734/bbj/2016/26488.
Dewanti AP, Azzahra F. Uji Karakteristik Sediaan Gel Ekstrak Etanol Daun Pandan (Pandanus Amaryllifolius
Roxb.) Dengan Basis Hydroxy Propyl Methyl Cellulose (Hpmc). AFAMEDIS. 2020 Nov 13;1(2):31-41.
Dewanti, N. I., & Sofia, F. F. (2017). Review Artikel: Aktivitas Farmakologi Ekstrak Daun Pandan Wangi
(Pandanus amaryllifolius Roxb.). Farmaka Suplemen, 15(2), 186-194.
Dewi, A. L., V. D. Siregar, and H. Kusumayanti. "Effect of Extraction Time on Tannin Antioxidant Level and
Flavonoid on Pandan Wangi Leaf (Pandanusamary Trifolium Roxb) Using Hydrothermal Extractor." Journal of
Physics: Conference Series. Vol. 1295. No. 1. IOP Publishing, 2019.
Ersel M, Uyanikgil Y, Akarca FK, Ozcete E, Altunci YA, Karabey F, Cavusoglu T, Meral A, Yigitturk G, Cetin
EO. Effects of silk sericin on incision wound healing in a dorsal skin flap wound healing rat model. Medical
Science Monitor: International Medical Journal of Experimental and Clinical Research. 2016;22:1064.
Ismail, H. F., Hashim, Z., Soon, W. T., Ab Rahman, N. S., Zainudin, A. N., & Majid, F. A. A. (2017).
Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities, and toxicity on
cells and zebrafish embryo. Journal of traditional and complementary medicine, 7(4), 452-465
Jeong W, Yang CE, Roh TS, Kim JH, Lee JH, Lee WJ. Scar prevention and enhanced wound healing induced by
polydeoxyribonucleotide in a rat incisional wound-healing model. International journal of molecular sciences.
Aug;18(8):1698.
International Journal of Health and Pharmaceutical
Lister IN, Amiruddin HL, Fachrial E, Girsang E. Anti-Aging Effectiveness of Avocado Peel Extract Ointment
(Persea Americana Mill.) against Hydration, Collagen, and Elasticity Levels in Wistar Rat. Journal of
Pharmaceutical Research International. 2021 Jun 22:173-84.
Lolok, N., Yuliastri, W. O., & Abdillah, F. A. (2020). Efek Antidiabetes Kombinasi Ekstrak Etanol Daun
Pandan Wangi (Pandanus amaryllifolius Roxb.) Dan Daun Salam (Syzygium polyanthum Wight.) Pada Tikus
Putih Dengan Metode Induksi Aloksan. Jurnal Mandala Pharmacon Indonesia, 6(01), 13-29.
Louis, H. et al. (2017) 'Determination of total phenolic content and some selected metals in extracts of Moringa
oleifera, Cassia tora, Ocimum gratissimum, Vernonia Baldwin and …', World News of Natural …, 11, pp. 11–
Nagar HK, Srivastava AK, Srivastava R, Kurmi ML, Chandel HS, Ranawat MS. Pharmacological investigation
of the wound healing activity of Cestrum nocturnum (L.) ointment in Wistar albino rats. Journal of
Pharmaceutics. 2016;2016.
Nanda Y, Salim MN, Iskandar CD. Histopatologi Kulit Mencit (Mus musculus) Fase Remodeling Pada
Penyembuhan Luka Sayat dengan Salep Getah Jarak Pagar (Jatropha curcas Linn). Jurnal Ilmiah Mahasiswa
Veteriner. 2017 Oct 12;1(4):780-7.
Nasiri E, Hosseinimehr SJ, Azadbakht M, Akbari J, Enayati-Fard R, Azizi S, et al. The healing effect of arnebia
euchroma ointment versus silver sulfadiazine on burn wounds in Rat. World J Plastic Surg. 2015;4(2):134–44.
Ningrum, Andriati & nguyen minh, Nhut & Schreiner, Matthias. (2015). Carotenoids and Norisoprenoids as
Carotenoid Degradation Products in Pandan Leaves ( Pandanus amaryllifolius Roxb.). International Journal of
Food Properties. 18. 1905-1914. 10.1080/10942912.2014.971186.
Nofikasari I, Rufaida A, Aqmarina CD, Failasofia F, Fauzia AR, Handajani J. Efek aplikasi topikal gel ekstrak
pandan wangi terhadap penyembuhan luka gingiva. Majalah Kedokteran Gigi Indonesia. 2016;2(2):53-9.
Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound healing: a cellular perspective. Physiological
reviews. 2019 Jan 1;99(1):665-706.
Rokhmah, N. N., Yulianita, Y., & Putra, R. A. (2021). EFEKTIVITAS GEL DAUN PANDAN WANGI
SEBAGAI OBAT LUKA BAKAR PADA TIKUS PUTIH JANTAN. Pharmacoscript, 4(2), 131-140.
Safitri, T. O., Nasution, A. N., Ginting, C. N., & Girsang, E. (2021, July). The Effectiveness of Red Spinach
Extract Ointment (Amaranthus tricolor L.) against Healing Second Degree Burns in Wistar Rat. In 2021 IEEE
International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce) (pp. 1-
. IEEE.
Saifudin M, Herbani M, Andriana D. PERBANDINGAN EFEK PERASAN DAGING DAUN LIDAH BUAYA
(Aloe vera L.) DAN POVIDONE IODINE TERHADAP EKSPRESI VEGF DAN JUMLAH LUMEN
PEMBULUH DARAH TIKUS WISTAR DENGAN LUKA SAYAT. Jurnal Kedokteran Komunitas. 2020 Jan
;8(1).
Shakya P, Sharma AK, Kumar N, Vellachi R, Mathew DD, Dubey P, et al. Bubaline cholecyst derived
extracellular matrix for reconstruction of full-thickness skin wounds in rats. Scientifica. 2016;2(1):1–13.
Shamanic, A. et al. "Hepatoprotective effects of Pandanus amaryllifolius against carbon tetrachloride (CCl4)
induced toxicity: A biochemical and histopathological study." Arabian Journal of Chemistry 14.10 (2021):
Silalahi M. Pandanus amaryllifolius Roxb (Pemanfaatan dan potensinya sebagai pengawet makanan). Jurnal
Pro Life. 2018;5(3):626-36.
Sugihartini N, Nuryanti E. Formulasi Krim Ekstrak Daun Kelor (Moringa oleifera) sebagai Sediaan Antiaging
(Formulation Cream of Extract Moringa oleifera Leave as Antiaging). Berkala Ilmu Kesehatan Kulit Dan
Kelamin. 2017;29(1):1-7.
Suryani CL, Murti ST, Ardiyan A, Setyowati A. Aktivitas Antioksidan Ekstrak Etanol Daun Pandan (Pandanus
amaryllifolius) dan Fraksi-Fraksinya. Agritech. 2017;37(3):271-9.
Suwannakul S, Chaibenjawong P, Suwannakul S. Antioxidant Anti-Cancer and Antimicrobial Activities of
Ethanol Pandanus amaryllifolius Roxb. leaf extract (In Vitro)-A potential medical application. Journal of
International Dental & Medical
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